Abstract

ABSTRACT The antibacterial activity of acetone, methanol, and water-soluble extracts of both leaf and stem of Barleria lupulina were evaluated against some human pathogens viz., Salmonella typhi, Escherichia coli, Pseudomonas aureginosa, Klebsiella pneumonia and Staphylococcus aureus. The highest antibacterial activities were exhibited at 100% concentrationof all the extracts against the bacterial pathogens. Acetone-soluble leaf (AL) and acetone-soluble stem (AS) extracts caused the maximum zone of inhibition against P. aureginosa, and methanol-soluble leaf (ML) and methanol-soluble stem (MS) extracts against of Salmonella typhi. However, Klebsiella pneumonia was inhibited by all the extracts. The result of HPTLC analysis revealed the presence of alkaloid having different phyto-constituents having their Rf values. Twenty phyto-constituents were identified from all the extracts in which the major compounds were methyl ester, phytol, benzoic acid and propenoic acid as per GC-MS analysis.