EFFECT OF CULTURAL CONDITIONS ON ETHANOL PRODUCTION BY LOCALLY ISOLATED SACCHAROMYCES CEREVISIAE BIO

Abstract

The present study describes the ethanol fermentation from sugarcane molasses by locally isolated yeast strain. Ten yeast strains were isolated from soil and cultured in 15% molassess medium. Saccharomyces cerevisiae Bio-07 gave maximum productivity (52.0g/L). Fermentation conditions were optimized for maximum production of ethanol. Maximum yield of ethanol (76.8 g/L) was obtained with 15% molasses concentration, 3% inoculum size, pH 4.5 and temperature 30 º C. Potassium Ferrocyanide (150ppm) was used to control the trace metals present in the molasses medium.